Membrane:Article Title: Unveiling the consequences of early human saliva contamination on membranes for guided bone regeneration.
Article Snippet: .. cytotoxicity before and after membrane saliva contamination The human gingival fibroblast cell line HGF- 1 (ATCC CRL- 2014, Manassas, USA) was grown in Dulbecco's modified Eagle's medium supplemented with 10% FBS and 1% penicillin/streptomycin solution (Gibco Life Technologies, Carlsbad, California, USA) in a 37°C humidified incubator with 5% CO2. .. HGF- 1 were seeded at a density of 40 000 cells/mL on the surface of the membrane disks (n = 3 per membrane group/per saliva donor) juxtaposed in Nunc non- adherent 48- well plates (Thermo Fisher Scientific, Roskilde, Denmark) with 300 μL media.
Modification:Article Title: Unveiling the consequences of early human saliva contamination on membranes for guided bone regeneration.
Article Snippet: .. cytotoxicity before and after membrane saliva contamination The human gingival fibroblast cell line HGF- 1 (ATCC CRL- 2014, Manassas, USA) was grown in Dulbecco's modified Eagle's medium supplemented with 10% FBS and 1% penicillin/streptomycin solution (Gibco Life Technologies, Carlsbad, California, USA) in a 37°C humidified incubator with 5% CO2. .. HGF- 1 were seeded at a density of 40 000 cells/mL on the surface of the membrane disks (n = 3 per membrane group/per saliva donor) juxtaposed in Nunc non- adherent 48- well plates (Thermo Fisher Scientific, Roskilde, Denmark) with 300 μL media.
Article Title: Dehydroleucodine Induces ROS-Mediated Mitochondrial Apoptosis and G2/M Cell Cycle Arrest in Oral Squamous Cell Carcinoma
Article Snippet: .. Human oral squamous cell carcinoma cell lines HSC3 (Merck KGaA, Darmstadt, Germany) and SCC4 (Bioresource Collection and Research Center, Hsinchu, Taiwan) as well as the normal human gingival fibroblast cell line HGF-1 (ATCC, Manassas, VA, USA) were cultured in high-glucose Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Waltham, MA, USA) and 1% penicillin-streptomycin solution (100 U/mL penicillin and 100 μg/mL streptomycin; Thermo Fisher Scientific). ..
Article Title: Unveiling the consequences of early human saliva contamination on membranes for guided bone regeneration
Article Snippet: .. The human gingival fibroblast cell line HGF‐1 (ATCC CRL‐2014, Manassas, USA) was grown in Dulbecco's modified Eagle's medium supplemented with 10% FBS and 1% penicillin/streptomycin solution (Gibco Life Technologies, Carlsbad, California, USA) in a 37°C humidified incubator with 5% CO 2 . .. HGF‐1 were seeded at a density of 40 000 cells/mL on the surface of the membrane disks ( n = 3 per membrane group/per saliva donor) juxtaposed in Nunc non‐adherent 48‐well plates (Thermo Fisher Scientific, Roskilde, Denmark) with 300 μL media.
Incubator:Article Title: Unveiling the consequences of early human saliva contamination on membranes for guided bone regeneration.
Article Snippet: .. cytotoxicity before and after membrane saliva contamination The human gingival fibroblast cell line HGF- 1 (ATCC CRL- 2014, Manassas, USA) was grown in Dulbecco's modified Eagle's medium supplemented with 10% FBS and 1% penicillin/streptomycin solution (Gibco Life Technologies, Carlsbad, California, USA) in a 37°C humidified incubator with 5% CO2. .. HGF- 1 were seeded at a density of 40 000 cells/mL on the surface of the membrane disks (n = 3 per membrane group/per saliva donor) juxtaposed in Nunc non- adherent 48- well plates (Thermo Fisher Scientific, Roskilde, Denmark) with 300 μL media.
Cell Culture:Article Title: Dehydroleucodine Induces ROS-Mediated Mitochondrial Apoptosis and G2/M Cell Cycle Arrest in Oral Squamous Cell Carcinoma
Article Snippet: .. Human oral squamous cell carcinoma cell lines HSC3 (Merck KGaA, Darmstadt, Germany) and SCC4 (Bioresource Collection and Research Center, Hsinchu, Taiwan) as well as the normal human gingival fibroblast cell line HGF-1 (ATCC, Manassas, VA, USA) were cultured in high-glucose Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Waltham, MA, USA) and 1% penicillin-streptomycin solution (100 U/mL penicillin and 100 μg/mL streptomycin; Thermo Fisher Scientific). ..
Article Title: Investigating the Effects of Heated Tobacco Products on Periodontal Healing: Insights From In Vivo and In Vitro Experiments
Article Snippet: .. Cell culture The human gingival fibroblast cell line HGF-1 (ATCC CRL-2014; American Type Culture Collection, Manassas, VA, USA) was used for in vitro experiments. .. Cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM; ATCC 30-2002, American Type Culture Collection) supplemented with 10% fetal bovine serum (Gibco, Invitrogen, Carlsbad, CA, USA), 100 U/mL penicillin, and 100 μg/mL streptomycin (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) at 37°C in a humidified atmosphere containing 5% CO 2 until confluence or subconfluence.
In Vitro:Article Title: Investigating the Effects of Heated Tobacco Products on Periodontal Healing: Insights From In Vivo and In Vitro Experiments
Article Snippet: .. Cell culture The human gingival fibroblast cell line HGF-1 (ATCC CRL-2014; American Type Culture Collection, Manassas, VA, USA) was used for in vitro experiments. .. Cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM; ATCC 30-2002, American Type Culture Collection) supplemented with 10% fetal bovine serum (Gibco, Invitrogen, Carlsbad, CA, USA), 100 U/mL penicillin, and 100 μg/mL streptomycin (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) at 37°C in a humidified atmosphere containing 5% CO 2 until confluence or subconfluence.
Article Title: Full-laser-enabled clean hierarchical structuring and multifunctional synergy for high-performance in vivo 3D-printed implants
Article Snippet: .. The mouse calvarial preosteoblast cell line MC3T3-E1 (ATCC CRL-2593, RRID:CVCL_5440) and the human gingival fibroblast cell line HGF-1 (ATCC CRL-2014, RRID:CVCL_3710) were used to evaluate the in vitro biocompatibility and osteogenic performance of the laser-treated titanium surfaces. .. Both cell lines were obtained from the American Type Culture Collection (ATCC) and are widely accepted as standard models for assessing osteoblast differentiation and soft tissue response, respectively, in implant-related studies.
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